Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Boxuan Zeng
Date created: 2011-10-13 11:00:00
Date modified: 2015-05-08 01:12:42
Tor with mutation L1415P in yeast vector pDHBtor
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K563056_sequence (Version 1) |
Description
We insert Tor to the modified plasmid pDHBtor. It can express protein Tor with mutation L1415P, when transform the plasmid into strain without normal Leu2 gene. The yeast with the plasmid will grow better in YPD containing multi-inhibitor than the yeast without the plasmid.Notes
The plasmid contains kanR, pBS ori, CEN/ARS, LEU2, and Tor with L1415P mutation.Source
Gene Tor is from BBa_K563011.| Sequence Annotation | Location | Component / Role(s) |
| Tor2 with L1415P mutation pBS ori KanR Leu2 CEN/ARS | 235,8365 13039,13706 12066,12738 9354,11564 13828,14367 | CDS feature/cds feature/misc sequence_feature CDS feature/cds CDS feature/cds feature/misc sequence_feature |