BBa_K564001

BBa_K564001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K564001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: S??bastien Lemire

Date created: 2011-09-18 11:00:00

Date modified: 2015-05-08 01:12:42

Prha: Rhamnose->PoPS

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K564001_sequence (Version 1)

Description

This part contains the rhamnose promoter and its two coregulators, rhaS and rhaR. This promoter is activated in the presence of L(+)-Rhamnose and inhibited by glucose due to catabolite repression. It can be used in a very analogous way to the arabinose promoter (i0500).

Notes

This part had an EcoRI site that needed to be eliminated. No issues during cloning.

Source

The DNA was obtained through two overlapping PCRs from the genomic DNA of Salmonella enterica serovar typhimurium strain LT2.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
+1	2102,2102	promoter, feature/promoter
-10	2088,2095	promoter, feature/promoter
RhaS	2055,2068	non_covalent_binding_site, feature/binding
rhaR	1,849	CDS, feature/cds
rhaS	1002,1838	CDS, feature/cds
PrhaS	1898,1911	feature/promoter, promoter
crp	1973,1986	non_covalent_binding_site, feature/binding
crp	2004,2017	feature/binding, non_covalent_binding_site
RhaS	2023,2036	feature/binding, non_covalent_binding_site
RhaS	2055,2068	feature/binding, non_covalent_binding_site
RhaR	1898,1911	feature/binding, non_covalent_binding_site
RhaR	1931,1944	non_covalent_binding_site, feature/binding

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K564001/1

Member of these Collections

• iGEM Parts Registry