BBa_K564021

BBa_K564021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K564021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: S??bastien Lemire

Date created: 2011-09-20 11:00:00

Date modified: 2015-05-08 01:12:42

aTC regulated promoter module: aTC--PoPS

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K564021_sequence (Version 1)

Description

This part contains the tet regulated promoter R0040 as well as the wild-type tetR gene organized opposite one another to allow creation of an autonomous tet regulatable promoter module.
Indirect evidence suggests it does and works as expected (induced by anhydrotetracycline).

Notes

DNA was obtained by gene synthesis and a mutation within the -10 of the promoter driving expression of tetR makes it unsure whether it is produced or not by this part.

Source

DNA synthesis (integrated DNA technology)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
B0015	1,123	engineered_region, feature/BioBrick
tetR	138,761	feature/misc, sequence_feature
B0032	769,781	feature/rbs, ribosome_entry_site
J23108 (-10 mutant)	790,825	feature/promoter, promoter
R0040	864,918	promoter, feature/promoter

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K564021/1

Member of these Collections

• iGEM Parts Registry