BBa_K565001

BBa_K565001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K565001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Juan Ricardo Ortiz Meneses

Date created: 2011-09-16 11:00:00

Date modified: 2015-05-08 01:12:42

Colicin G gene cluster

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K565001_sequence (Version 1)

Description

Colicin G is a protein produced by Escherichia coli CA46 strain. They make holes in bacterial membranes, being lethal for related strains of E. coli.

The Colicins G proteins have specific activity, they need the presence of Fiu protein in the outer membrane of target cells in order to recognize them, and use the TonB/ExbB, D translocation route to slip in the cell and lyse it.

In our project, we used Colicin G to kill pathogen cells (in our case they were E. coli DH5alpha strain) by membrane lysis, desinfecting the contaminated water.

Notes

Please see Patzer et al. Microbiology (2003), 149, 2557???2570 for info on this point

Source

The sequence of the colicin G determinants have been deposited under the accession numbers AJ515251, in the EMBL/GenBank database.
A good reference paper on this part is: Patzer et al. Microbiology (2003), 149, 2557???2570
This part was central in our 2011 iGEM project: http://2011.igem.org/Team:Valencia

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K565001/1

Member of these Collections

• iGEM Parts Registry