CFP opt

BBa_K566003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K566003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Rodriguez
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:42

CFP optimized for E. coli



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_K566003_sequence (Version 1)

    Description

    Cyan fluorescent protein optimized for expression in E. coli.

    It was optimized using preferential codons, originally taken from BBa_E0022

    Notes

    Preferential codons were implemented for improving expression in E. coli.

    Analysis of illegal sites was made, they were found and removed.

    Source

    Synthesized DNA.

    Sequence Annotation Location Component / Role(s)
    Optimized CFP
    Stop
    Initial codon
    SsrA
    		1,762
    759,762
    1,3
    718,758
    		feature/cds CDS
    feature/stop stop_codon
    start_codon feature/start
    tag feature/tag

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K566003/1
     

    Attachments

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