Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Rodriguez
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:43
PenI repressor optimized for E. coli (inverted sequence)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K566010_sequence (Version 1) |
Description
PenI repressor with codon optimization for improving expression into E. coli.This protein represents a negative repressor for pPenI promoter (BBa_R0074).
Sequence was inverted for simplifying DNA synthesis.
Notes
Search for illegal sites was performed, they were found and eliminated.Source
Original sequence was taken from Registry (BBa_C0074), this was optimized with preferential codons for better expression in E. coli.| Sequence Annotation | Location | Component / Role(s) |
| PenI repressor Stop codon Start codon | 1,423 1,6 421,423 | feature/cds CDS feature/stop stop_codon start_codon feature/start |