RFP opt

BBa_K566013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K566013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Rodriguez

Date created: 2011-09-25 11:00:00

Date modified: 2015-05-08 01:12:43

RFP optimized for E. coli (+LVA)

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K566013_sequence (Version 1)

Description

RFP optimized with preferential codons for better expression into E. coli.

Includes LVA tag.

Notes

Sequence was design considering use of preferential codons, as well, a complete search looking for illegal sites for classic BioBricks was performed, they were found and deleted.

Source

Sequence was taken from BioBrick BBa_J06505, then optimized and synthesized.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mCherry (RFP)	1,708	feature/cds, CDS
LVA	709,717	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K566013/1

Member of these Collections

• iGEM Parts Registry