Cph8

BBa_K566025 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K566025
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Rodriguez
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:43

Cph8 CDS



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K566025_sequence (Version 1)

Description

The red light-sensing protein Cph8 is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor

Notes

For the project that this part was made, it was needed to use other Assambly Standard.

Source

Extracted by PCR directly from pCph8 plasmid of Dr. Jeff Tabor.

Sequence Annotation Location Component / Role(s)
Cph8
1,2235
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K566025/1