BBa_K567001

BBa_K567001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K567001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chunying Li and Yunfeng Ruan
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:43

lacI-Ptrc-tRNA(Arg)



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K567001_sequence (Version 1)

    Description

    The tRNA(Arg) is under the control of promoter tac. tRNA(Arg) expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.

    Notes

    The correct processing to produce mature tRNA should be ensured.

    Source

    LacI- Ptac derived from plasmid pTrc99B. tRNA(Arg) from E.coli ArgW operon.

    Sequence Annotation Location Component / Role(s)
    PlacI
    lacI
    Ptrc
    tRNA-Arg
    rrnB TE
    		331,367
    397,1479
    1709,1739
    1785,1859
    2054,2133
    		feature/promoter promoter
    CDS feature/cds
    promoter feature/promoter
    sequence_feature feature/dna
    stem_loop feature/stem_loop

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K567001/1
     

    Attachments

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