BBa_K567003

BBa_K567003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K567003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yunfeng Ruan and Chunying Li

Date created: 2011-09-28 11:00:00

Date modified: 2015-05-08 01:12:43

Pbla-Luc-TAG

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K567003_sequence (Version 1)

Description

Luciferase under the control of β-lactamase promoter with TAG insertion. A TAG codon is inserted after the initial codon of the gene. This part is used to testify the function of corporation of PT7-TDRS (BBa_K567011) and tRNA(Asp)-TAG (BBa_K567013).

Notes

Point mutation is used to obtain this part from the wild type.

Source

β-lactamase promoter is derived from pUC18 β-lactamase operon. Wild type luciferase is derived from BBa_I712019.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG	287,289	start_codon, feature/start
luciferase-TAG	287,1942	feature/cds, CDS
TAG	290,292	feature/mutation, sequence_alteration
Pbla	217,244	promoter, feature/promoter
rbs	278,282	ribosome_entry_site, feature/rbs

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K567003/1

Member of these Collections

• //cds/enzyme
• iGEM Parts Registry