BBa_K567017

BBa_K567017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K567017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bin Zhao

Date created: 2011-09-29 11:00:00

Date modified: 2015-05-08 01:12:43

PT7-RFP-6AGG

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K567017_sequence (Version 1)

Description

RFP with 6 AGG-codon insertions after ATG under the control of T7 promoter and lac operator. The protein can be expressed successfully when co-transformed with PT7-TDRS (BBa_K567011) and tRNA(Asp)-AGG (BBa_K567012). Modified RFP exhibits lower red fluorescence brightness. This part is used to testify the function of PT7-TDRS (BBa_K567011).

Source

RFP derived from BBa_I13521.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
PT7	2,18	promoter, feature/promoter
lac operator	21,44	feature/operator, operator
rbs	75,81	feature/rbs, ribosome_entry_site
6AGG	152,169	sequence_alteration, feature/mutation
RFP-6AGG	89,844	CDS, feature/cds
T7 TE	935,982	feature/stem_loop, stem_loop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K567017/1

Member of these Collections

• iGEM Parts Registry