BBa_K576013

BBa_K576013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K576013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joon Hyuk Hong

Date created: 2011-06-19 11:00:00

Date modified: 2015-05-08 01:12:45

J23101-RBS-GFP-Transcription Terminator

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K576013_sequence (Version 1)

Description

In the positive control, GFP1 and GFP2 flank RFC53, which will not disrupt translation regardless of the linker. Therefore, fluorescence is expected. The experimental run will ideally show fluorescence resulting from the self-excision of IN1 and IN2.

Notes

Positive Control

Source

Positive Control

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K576005	1,535	feature/BioBrick, engineered_region
BBa_J23101	1,35	engineered_region, feature/BioBrick
BBa_B0034	44,55	engineered_region, feature/BioBrick
conserved	48,51	conserved_region, feature/conserved
GFP1	62,535	CDS, feature/cds
BBa_K576001	62,535	engineered_region, feature/BioBrick
GFP2	536,787	feature/cds, CDS
BBa_K576002	536,787	feature/BioBrick, engineered_region
BBa_K576006	536,924	feature/BioBrick, engineered_region
BBa_B0015	799,924	engineered_region, feature/BioBrick
stem_loop	827,870	stem_loop, feature/stem_loop

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K576013/1

Member of these Collections

• iGEM Parts Registry