BBa_K577890

BBa_K577890 Version 1

Component

This part has been discontinued.

Please consider using BBa_K577006 as a replacement.

Source: http://parts.igem.org/Part:BBa_K577890

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alberto Purwada

Date created: 2011-09-10 11:00:00

Date modified: 2015-05-08 01:12:46

pBad+YFPc

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K577890_sequence (Version 1)

Description

This reporter part is a combination of a pBad promoter (inducible with arabinose) and a YFP gene. The purpose of this part is to provide the user with a simple, but complete, inducible YFP genetic device. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. But it is important for the user to determine if his or her cell line contain AraC gene, since AraC gene is required to actually induce pBad promoter with arabinose. See BBa_K577884 or BBa_I13458 for more information.

Notes

There is no specific design consideration required.

Source

This part is obtained from ligating 2 biobricks, BBa_I13453 and BBa_E0430.

Components

Access	Instance	Definition
public	BBa_I13453	BBa_I13453
public	BBa_E0430	BBa_E0430

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13453	1,130	BBa_I13453
BBa_E0430	139,1016	BBa_E0430

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_K577006/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K577890/1

Member of these Collections

• iGEM Parts Registry