BBa_K582001

BBa_K582001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K582001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mitesh Agrawal

Date created: 2011-09-27 11:00:00

Date modified: 2015-05-08 01:12:46

Cas9 genome from the Streptococcus Thermophilus LMD-9 strain

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K582001_sequence (Version 1)

Description

Cas9 genome is a part of the CRISPR sequence existing in the Strep. Thermophilus LMD-9 strain. As referred by the Barrangou 2011 paper, cas9 is the only essential cas protein required for the CRISPR mechanism to express. We propose a biobrick with the cas9 site present in it as well as the promoter sigma factor 70 for its expression.

Notes

We had to mutate the restriction sites present in the cas9 sequence which are present in the biobrick.

Source

http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=graph&from=1379975&to=1384141

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• rdf-schema#seeAlso: Q03JI6
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K582001/1

Member of these Collections

• iGEM Parts Registry