BBa_K582002

BBa_K582002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K582002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mitesh Agrawal

Date created: 2011-09-27 11:00:00

Date modified: 2015-05-08 01:12:46

Complete Repeat/Spacer Region(1400 bp) of LMD 9

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K582002_sequence (Version 1)

Description

The Spacer/Repeat Region part has been extracted from the Streptococcus Thermophilus LMD9 genome sequence. If inserted along with the CRISPR protein system and sigma factor 70, we hypothesize that the spacer/repeat region should express itself and make bacteria resistant to which the spacer region is homologous.

Notes

The sequence has the restriction sites present on the pSB1C3 backbone. We will have to subject it to mutagenesis to make the biobrick functional.

Source

http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=graph&from=1379975&to=1384141 for Cas9

http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=genbank&from=648900&to=650325 for Repeat/Spacer Region

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K582002/1

Member of these Collections

• iGEM Parts Registry