Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Ignacio Maycotte Cervantes
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:47
RecA protein coding sequence
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K587001_sequence (Version 1) |
Description
Designed by Antonio Villarreal Larrauri and David Maycotte Cervantes.RecA protein that activates the expression of the promoters for arabinose concentration by reducing the activity of the lambda repressor.
RecA is a protein used for the cleavage of protein lambda. It has shown that it has a cleavage activity when its binded to lambda repressor (Sauer et al, 1981). This part represents a good form to inhibit the activity of lambda repressor in construct where lambda repressor and the lambda operators appear.
Notes
The sequence of RecA was changed from its original source, because there was a EcoRI site in the middle of the DNA sequence so the sequence chaged in one base to modify that restriction site.Source
The information of this sequence comes from the article:Cleavage of the X and P22 Repressors by recA Protein by Robert T. Sauer, Michael J. Ross, and Mark Ptashnel from the Journal of Biological Chemistry. Obtained from the Escherichia coli CFT073 chromosome, complete genome from NCBI