BBa_K590014

BBa_K590014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K590014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Brasino, Alicia Wong, Rashmi Ravichandran
Date created: 2011-09-14 11:00:00
Date modified: 2015-05-08 01:12:47

pGA3K3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert



    • Details

    Types
    DnaRegion

    Roles
    Plasmid_Backbone

    plasmid_vector

    Sequences BBa_K590014_sequence (Version 1)

    Description

    Backbone that is Gibson reaction friendly (Bgl Brick)

    Notes

    sequence confirmed

    Source

    Made from Gibson reaction

    Sequence Annotation Location Component / Role(s)
    pGA suffix
    E. coli his operon terminator
    terminator stem loop
    Vr
    kanR
    p15A ori
    Vf2
    terminator stem loop
    pGA prefix
    		1,21
    22,93
    30,64
    157,176
    239,1054
    1386,2210
    2611,2630
    2700,2719
    2729,2749
    		engineered_region feature/BioBrick
    sequence_feature feature/misc
    stem_loop feature/stem_loop
    primer_binding_site feature/primer_binding
    feature/cds CDS
    feature/cds CDS
    primer_binding_site feature/primer_binding
    stem_loop feature/stem_loop
    sequence_feature feature/misc

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K590014/1
     

    Attachments

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