BBa_K590014

BBa_K590014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K590014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Brasino, Alicia Wong, Rashmi Ravichandran
Date created: 2011-09-14 11:00:00
Date modified: 2015-05-08 01:12:47

pGA3K3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K590014_sequence (Version 1)

Description

Backbone that is Gibson reaction friendly (Bgl Brick)

Notes

sequence confirmed

Source

Made from Gibson reaction

Sequence Annotation Location Component / Role(s)
pGA suffix
E. coli his operon terminator
terminator stem loop
Vr
kanR
p15A ori
Vf2
terminator stem loop
pGA prefix
1,21
22,93
30,64
157,176
239,1054
1386,2210
2611,2630
2700,2719
2729,2749
engineered_region feature/BioBrick
sequence_feature feature/misc
stem_loop feature/stem_loop
primer_binding_site feature/primer_binding
feature/cds CDS
feature/cds CDS
primer_binding_site feature/primer_binding
stem_loop feature/stem_loop
sequence_feature feature/misc
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K590014/1