| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K592000_sequence (Version 1)
|
Description
This part is identical to BBa_I15010, the cph8 red light sensor. We were unable to transform BBa_I15010 from the the 2011 iGEM distribution kit. PCR attempts using VF2 and VR failed to amplify any DNA from the well sample. In the end, we chose to amplify cph8 from Jeffrey Tabor's pJT122 plasmid provided by Chris Voigt. The cph8 in pJT122 contained an internal PstI site, which was removed by point-mutagenesis. The resultant DNA sequence is identical to that of BBa_I15010.
Notes
The source DNA (pJT122) contained illegal restriction site (PstI) that had to be removed.
Source
Jeffrey Tabor's pJT122 plasmid
