Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Sun
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:12:48
YF1 blue-light sensor protein
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K592004_sequence (Version 1) |
Description
YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.Notes
The sequence information was provided in Moglich and Moffat's article. The physical DNA was synthesized by GenScript. Codon-optimization may enhance its expression.Source
Ytva comes from the genome of Bacillus subtilis.FixL derives from the genome of Bradyrhizobium japonicum.