Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Sun
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:49
HO1-pcyA, no promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K592013_sequence (Version 1) |
Description
This is the PCB chromophore module without promoter, with B1001 terminator.*Heme oxygenase and phycocyanobilin:ferredoxin oxidoreductase
*Used for light sensing system
*Recommend weak promoters
*B1001 terminator instead of B0015, lambda-red compatible
Notes
B1001 instead of B0015 since this part was chosen to put into the bacterial chromosome using lambda-red recombination. The standard double terminator B0015 has extensive homologous sites within E.coli genome, therefore it's not used.This part lacks promoter. What promoter to be used is up to the user. However, we recommend weak promoters due to HO1's enzymatic activity kills cells by consuming heme.
Source
Assembled using parts from the MIT 2011 iGEM distribution.| Access | Instance | Definition |
| public public public public public | BBa_B0034 BBa_I15008 BBa_B0034 BBa_I15009 BBa_B1001 | BBa_B0034 BBa_I15008 BBa_B0034 BBa_I15009 BBa_B1001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_I15008 BBa_B0034 BBa_I15009 BBa_B1001 | 1,12 19,769 778,789 796,1570 1579,1612 | BBa_B0034 BBa_I15008 BBa_B0034 BBa_I15009 BBa_B1001 |