Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Sun
Date created: 2011-09-17 11:00:00
Date modified: 2016-10-14 06:58:34
amilCP Blue Coding Device
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K592015_sequence (Version 1) |
Description
This neatly designed color coding cassette is a prime candidate to replace BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacteria colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized.Promoter is constitutive, the RBS is strong, the double terminator is not B0015 but B0014.
Notes
B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times.Source
amilCP comes from the genome of Acropora millepora. All other parts are assembled from the iGEM 2011 distribution kit.| Access | Instance | Definition |
| public public public public | BBa_J23101 BBa_B0034 BBa_K592009 BBa_B0014 | BBa_J23101 BBa_B0034 amilCP BBa_B0014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 BBa_B0034 BBa_K592009 BBa_B0014 | 1,35 44,55 62,730 739,833 | BBa_J23101 BBa_B0034 amilCP BBa_B0014 |