Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Sun
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:49
PFixK2-lambda C1-B0034-amilCP blue light sensor output
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K592020_sequence (Version 1) |
Description
FixK2 promoter is the downstream signaling promoter of YF1/FixJ blue light-sensing system. The YF1/FixJ system become "inactive" when illuminated by blue light, and "active" in darkness. Needless to say, a system that works in the opposite way with lights activating and darkness inactivating is more desirable. Therefore, an inverter was placed behind FixK2 promoter. When in dark, YF1 becomes phosphorylated and activates FixJ, its own RR. FixJ in turn binds to FixK2 promoter, allowing the transcription of the lambda C1, thus repressing the amilCP output. In light however, YF1/FixJ has no effect on FixK2 promoter, which in turn has near-zero activity. The lambda C1 concentration drops, eventually amilCP will be produced.Notes
The Q04510 inverter contains B0015 double terminator which gives unspecific primer binding of VR. Touchdown PCR was required to amplify the sequence for screening.Source
FixK2 promoter: Bradyrhizibium japonicumamilCP: Acropora millepora
| Access | Instance | Definition |
| public public public public | BBa_K592006 BBa_Q04510 BBa_B0034 BBa_K592009 | BBa_K592006 BBa_Q04510 BBa_B0034 amilCP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K592006 BBa_Q04510 BBa_B0034 BBa_K592009 | 1,250 259,1245 1254,1265 1272,1940 | BBa_K592006 BBa_Q04510 BBa_B0034 amilCP |