BBa_K592020

BBa_K592020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K592020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Sun
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:49

PFixK2-lambda C1-B0034-amilCP blue light sensor output



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K592020_sequence (Version 1)

Description

FixK2 promoter is the downstream signaling promoter of YF1/FixJ blue light-sensing system. The YF1/FixJ system become "inactive" when illuminated by blue light, and "active" in darkness. Needless to say, a system that works in the opposite way with lights activating and darkness inactivating is more desirable. Therefore, an inverter was placed behind FixK2 promoter. When in dark, YF1 becomes phosphorylated and activates FixJ, its own RR. FixJ in turn binds to FixK2 promoter, allowing the transcription of the lambda C1, thus repressing the amilCP output. In light however, YF1/FixJ has no effect on FixK2 promoter, which in turn has near-zero activity. The lambda C1 concentration drops, eventually amilCP will be produced.

Notes

The Q04510 inverter contains B0015 double terminator which gives unspecific primer binding of VR. Touchdown PCR was required to amplify the sequence for screening.

Source

FixK2 promoter: Bradyrhizibium japonicum
amilCP: Acropora millepora

igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K592020/1