BFP

BBa_K592100 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K592100
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Gullberg
Date created: 2011-09-19 11:00:00
Date modified: 2016-01-25 04:45:27

Blue Fluorescent Protein (mTagBFP)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K592100_sequence (Version 1)

Description

This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm. It has a tyrosine-based chromophore, giving it substantially higher brightness, faster chromophore maturation and higher pH stability than blue fluorescent proteins with a histidine in the chromophore. Subach et. al. started with a red fluorescent protein (TagRed) and converted it to a bright blue monomeric protein. See the article listed in source. The sequence has been codon optimized for expression in E coli by DNA 2.0.

Notes

The sequence has been codon optimized for expression in E coli by DNA 2.0.

Source

Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.

Sequence Annotation Location Component / Role(s)
mTagBFP
1,699
CDS feature/cds
igem#experience Works  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K592100/1