BBa_K594000

BBa_K594000 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K594000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yu Xiaoyan

Date created: 2011-09-29 11:00:00

Date modified: 2015-05-08 01:12:49

a luxI homologous AHL synthetase from Sinorhizobium meliloti 1021

Details

• Types: DnaRegion
• Roles: Coding; CDS

Description

The S. meliloti strain Rm1021 harbors at least two quorum-sensing systems; one of them is sinR/sinI system, The sinI is responsible for the production of several novel AHLs, including C12-HSL, 3-Oxo-C14-HSL, C16:1-HSL, 3-Oxo-C16:1-HSL, C18-HSL, some of theses are the longest AHLs identified so far. The sin system correlates with a delay in the appearance of nitrogen-fixing nodules, as well as with an overall decrease in the number of pink nodules, suggesting a role for quorum sensing in establishing a successful symbiosis with Medicago sativa.

As a autoinducer synthatase, the use of sinI is the same with luxI, namely to produce AHLS.

Notes

The Sinorhizobium meliloti 1021 is a mode organism in Rhizobia. so We got the S. meliloti 1021 from Nanjing Agriculture University easily, and then cloned the sinI gene from S. meliloti 1021 with primers designed by the part design tool in the website ginkgobioworks.com.

Source

SinI is from the Sinorhizobium meliloti 1021. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K594000/1

Member of these Collections

• iGEM Parts Registry