BBa_K598002

BBa_K598002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K598002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yan GONG, Shuobin CHEN, Yunpeng LIU, Xiaowei YAN

Date created: 2011-09-26 11:00:00

Date modified: 2015-05-08 01:12:50

Bistable Switch Mutant 68

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K598002_sequence (Version 1)

Description

RFP+RBS_SDA-C1+OR21 double directed promoter+SDcro CI434+eGFP

Notes

mutate the RBS seqence change the translation rate of CI434 to get the appropriate ratio of green and red.

Source

The part is made up of the standard parts from partsregistry, and some of the sub-parts are inverted by PCR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mRFP	1,681	feature/cds, CDS
RBS	682,693	ribosome_entry_site, feature/rbs
lamda CI repressor	708,1421	CDS, feature/cds
RBS	1422,1433	ribosome_entry_site, feature/rbs
PRM (CI434 regulated promoter)	1440,1491	promoter, feature/promoter
PR (CI regulated promoter)	1492,1540	feature/promoter, promoter
RBS	1556,1567	ribosome_entry_site, feature/rbs
CI434 repressor	1568,2203	CDS, feature/cds
RBS	2214,2225	feature/misc, sequence_feature
eGFP	2226,2945	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K598002/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/bidirectional
• //plasmidbackbone/copynumber/high
• iGEM Parts Registry