Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cyrille Pauthenier
Date created: 2011-07-24 11:00:00
Date modified: 2015-05-08 01:12:51
Multi-host replicative plasmid, for B. Subtilis and E. Coli
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K606003_sequence (Version 1) |
Description
This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).This plasmid is ideal for importing parts in Subtilis: all the cloning can be done in E. Coli, as well as the amplification. Once the plasmid is extracted, you can incorporate it directly in B. Subtilis using electroporation.
This plasmid is Ampicilyn resistant in E. Coli, and is tetracycline resistant in both B. Subtilis and E. Coli.
The biobrick BBa_J04450 is inserted between the E X and SP site, has a negative cloning control in E. Coli
Notes
The EcoRI site has to be eliminated and the Biobricks prefixes and suffixes were recoveredSource
The plasmid was obtained from Harald Putzer laboratory at the IBPC http://www.ibpc.fr/UPR9073/equipe_HaraldJackie/AccueilJAP_HPGB.htm . This is the fusion of the commercial plasmids pBC16-1 and pTZ19R via the site EcoRI. This site has been eliminated by the 2011 iGEM Paris Team by quick change mutagenesis, and the multicloning site was transformed into the biobrick standards.| Sequence Annotation | Location | Component / Role(s) |
| Biobrick EX pBC16-1 plasmid (B.S. plasmid) Tetracycline resistance gene DNA minor replication origin OriL DNA: Major Replication origin OriU LacZ alpha disabled by the cloning SYNPTZ19R Beta lactamase peptide (ampicilin) Replication origin for E. Coli pT7 promoter Biobrick SP | 1,21 22,3072 80,1468 2120,2398 2813,2838 3076,3244 3078,5904 3835,4692 4867,5469 5871,5893 5905,5924 | feature/BioBrick engineered_region feature/misc sequence_feature CDS feature/protein sequence_feature feature/misc feature/misc sequence_feature CDS feature/protein sequence_feature feature/misc feature/protein CDS feature/misc sequence_feature feature/promoter promoter engineered_region feature/BioBrick |