| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K607030_sequence (Version 1)
|
Description
LasR protein with moderately fast degradation tag under transcriptional control of PlasB promoter and reverse antisense PBad promoter, the RBS has a strength of 1.0 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.
Notes
none
Source
2011 distribution
