Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sophie Cramer
Date created: 2011-09-13 11:00:00
Date modified: 2015-05-08 01:12:52
IPTG-inducible Promoter with plastic binding domain-tagged GFP
| Types | DnaRegion |
| Roles | tag Tag |
| Sequences | BBa_K608404_sequence (Version 1) |
Description
During phage display experiments small sequences were found that bind to the polystyrene plastic surfaces of the used microtiter plates. The binding was strong enough to resist several washing steps. The sequence binds to plastic due to its high hydropathy.Notes
To make the plastic binding domain observable we coupled it to GFP as a reporter. Due to the high hydropathy of the plastic binding sequences this protein seems to be toxic to the bacteria. For this reason we used an inducible promoter for the expression of this part. The cells can be cultivated until an optical density of 60-80 followed by an addition of IPTG to induce the expression of the protein.Source
reference: "The nature of target-unrelated peptides recovered in the screeningof phage-displayed random peptide libraries with antibodies"
| Access | Instance | Definition |
| public public public | BBa_J04500 BBa_E0040 BBa_K608405 | BBa_J04500 GFP BBa_K608405 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_E0040 BBa_K608405 | 1,220 227,946 955,985 | BBa_J04500 GFP BBa_K608405 |