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BBa_K611015

BBa_K611015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K611015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nicholas Csicsery

Date created: 2011-09-17 11:00:00

Date modified: 2015-05-08 01:12:53

Tet Repressor and Promoter Wild Type Control

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K611015_sequence (Version 1)

Description

This part is can be used as a control for BBa_K611009 and BBa_K611012, which test either C0040 and R0040 mutants respectively.

Notes

There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.

Source

Built from existing parts in the registry though BioBrick assembly.

Components

Access	Instance	Definition
public public public public public public public	BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 BBa_I13453 BBa_B0034 BBa_C0040	p(tetR) BBa_B0034 GFP BBa_B0015 BBa_I13453 BBa_B0034 tetR

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 BBa_I13453 BBa_B0034 BBa_C0040	1,54 63,74 81,800 809,937 946,1075 1084,1095 1102,1786	p(tetR) BBa_B0034 GFP BBa_B0015 BBa_I13453 BBa_B0034 tetR