Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Timothy Fenton
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:53
Mutant Regulatory protein/promoter characterization plasmid
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K611018_sequence (Version 1) |
Description
This part can be used to characterize promoter/repressor and promoter/activator pairs by swapping in the desired parts on either end. To characterize promoter mutants, ligate the corresponding repressor or activator on the 3' end by cutting the plasmid with speI and pstI and the regulatory protein with XbaI and pstI. Then put the desired mutant promoter on the 5' end. Repressor/activator mutants can also be characterized by simply putting a wildtype promoter on the 5' end and adding the mutant repressor or activator on the 3' end. Expression is reported via GFP fluorescence. The amount of repressor/activator can be controlled using the arabinose inducible pBAD promoter. Transcriptional termination takes place within the psb1C3 plasmid which means a terminator does not need to be added after the inserted coding sequence is added.Notes
When a protein coding sequence is added, there is no need to add a terminator since termination happens in the psb1C3 plasmid.Source
This part comes entirely from parts already available in the Parts Registry.| Access | Instance | Definition |
| public public public public public | BBa_B0034 BBa_E0040 BBa_B0015 BBa_I13453 BBa_B0034 | BBa_B0034 GFP BBa_B0015 BBa_I13453 BBa_B0034 |