BBa_K613001

BBa_K613001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K613001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alessandro Ferrari, Vincent Zimmern, Nadine Guenat, Henrike Niederholtmeyer
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:54

T7 promoter family member



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K613001_sequence (Version 1)

Description

Parts K613001 through K613006 are T7 promoter variants. K613001 is the T7 promoter wildtype. The other sequences were derived by single base pair mutations that have been shown to alter its strength.

Notes

Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419???10430 (2000).

Source

synthetic

Sequence Annotation Location Component / Role(s)
RBS
T7 promoter
96,101
30,52
ribosome_entry_site feature/rbs
feature/promoter promoter
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K613001/1