Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alessandro Ferrari, Vincent Zimmern, Nadine Guenat, Henrike Niederholtmeyer
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:54
T7 promoter family member (LacI repressed)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K613009_sequence (Version 1) |
Description
Parts K613007 through K613012 are T7 promoter variants containing the lac operator just downstream of the T7 promoter. K613007 contains the T7 promoter wildtype sequence. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct.Notes
Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419???10430 (2000).Source
synthetic| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter lac operator RBS | 30,52 55,75 107,112 | feature/promoter promoter feature/operator operator ribosome_entry_site feature/rbs |