BBa_K613010

BBa_K613010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K613010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alessandro Ferrari, Vincent Zimmern, Nadine Guenat, Henrike Niederholtmeyer
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:54

T7 promoter family member (LacI repressed)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K613010_sequence (Version 1)

Description

Parts K613007 through K613012 are T7 promoter variants containing the lac operator just downstream of the T7 promoter. K613007 contains the T7 promoter wildtype sequence. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct.

Notes

Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419???10430 (2000).

Source

synthetic

Sequence Annotation Location Component / Role(s)
T7 promoter
lac operator
RBS
30,52
55,75
107,112
feature/promoter promoter
feature/operator operator
ribosome_entry_site feature/rbs
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K613010/1