BBa_K613015

BBa_K613015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K613015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nadine Guenat, Lilia Salimova

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:54

TetR E37A W43S T141A mutant

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K613015_sequence (Version 1)

Description

This is a TetR mutant, who carries the triple mutation EA37WS43TA141.

Notes

The mutations were introduced by site-directed mutagenesis.

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the mutations by site-directed mutagenesis.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
W43S	127,129	sequence_alteration, feature/mutation
E37A	109,111	feature/mutation, sequence_alteration
A141T	421,423	sequence_alteration, feature/mutation

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K613015/1

Member of these Collections

• iGEM Parts Registry