BBa_K613018

BBa_K613018 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K613018
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lilia Salimova
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:54

TetR Y42F K108E mutant



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K613018_sequence (Version 1)

Description

This is a TetR mutant, who carries the YF42KE108 mutation.

Notes

The mutation was introduced by site-directed mutagenesis

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the Y42F mutation by site-directed mutagenesis. This mutation had already been described by Krueger et al (2007).

Sequence Annotation Location Component / Role(s)
Y42F
K108E
124,126
322,324
feature/mutation sequence_alteration
feature/mutation sequence_alteration
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K613018/1