BBa_K613018

BBa_K613018 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K613018

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lilia Salimova

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:54

TetR Y42F K108E mutant

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K613018_sequence (Version 1)

Description

This is a TetR mutant, who carries the YF42KE108 mutation.

Notes

The mutation was introduced by site-directed mutagenesis

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the Y42F mutation by site-directed mutagenesis. This mutation had already been described by Krueger et al (2007).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Y42F	124,126	feature/mutation, sequence_alteration
K108E	322,324	feature/mutation, sequence_alteration

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K613018/1

Member of these Collections

• iGEM Parts Registry