BBa_K613023

BBa_K613023 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K613023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nadine Guenat

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:54

TetR E37A P39Q Y42F mutant

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K613023_sequence (Version 1)

Description

This is a TetR mutant with the EA37PQ39YF42 triple mutation

Notes

The mutation was introduced by site-directed mutagenesis

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the mutation by site-directed mutagenesis.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
E37A	109,111	sequence_alteration, feature/mutation
P39Q	115,117	feature/mutation, sequence_alteration
Y42F	124,126	feature/mutation, sequence_alteration
BBa_K613023	1,621	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K613023/1

Member of these Collections

• iGEM Parts Registry