Types | DnaRegion
|
Roles | chromosome
Cell
|
Description
The EcNR2 strain is a modified E. coli strain which has been designed for easy genome manipulation.
Notes
MutS KO using lambda red to replace gene with chloramphenicol cassette.
Source
Harris H. Wang, Farren J. Isaacs, Peter A. Carr, Zachary Z. Sun, George Xu, Craig R. Forest, George M. Church. Programming cells by multiplex genome engineering and accelerated evolution. (2009). Nature, 460(7257):894-8.