BBa_K619891

BBa_K619891 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K619891
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: BYU IGEM team
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:54

Mutated prfA thermosensor from listeria



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K619891_sequence (Version 1)

    Description

    This is the prfA-UTR thermosensor from Listeria monocytogenes randomly mutated to produce a thermosensor which gives increased expression when switching from 30 to 35 degrees celsius. This part was created by TAQ-mediated random mutagenesis of part BBa_K619889

    Notes

    The prfA-UTR parent contruct BBa-K619889 has a RBS which has been optimized for E. coli.

    Source

    mutated prfA-UTR

    Sequence Annotation Location Component / Role(s)
    rbs
    		113,118
    		feature/rbs ribosome_entry_site

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K619891/1
     

    Attachments

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