BBa_K619896

BBa_K619896 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K619896
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julianne Grose
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:54

Pcon lox gent double terminator lox



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K619896_sequence (Version 1)

Description

This construct contains a constitutive promoter (BBa_J23106) followed by gentamycin resistance surrounded by lox sites. This part will allow expression from a downstream reporter from the constitutive promoter when Cre is expressed. Thus, one can initiated expression of Cre from an inducible promoter, and have constitutive expression of a reporter and loss of gentamycin resistance.

Notes

indicated under sources.

Source

The Pcon comes from BBa_J23106, the gentamycin resistance flanked by lox sites was a gift from Dr. Joel Griffitts and was amplified from pJG181.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K619896/1