mdnE

BBa_K627005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K627005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM11 Potsdam_Bioware
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:56

ABC transporter from the mdn-cluster



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K627005_sequence (Version 1)

Description

The BioBrick mdnE is a part of the whole microviridin gene (mdn) cluster, which encodes the protease
inhibitor microviridin L. Microviridins are tricyclic depsipeptides, which are ribosomally synthesized
by Microcystis (Ziemert et al., 2010). They have a promising potential for therapy as they can block
disease-relevant proteases (Ziemert et al., 2008).



Microviridins are synthesized from a ribosomal precursor peptide (MdnA). Additionally, the microviridin L
biosynthesis gene cluster consists of genes encoding an ATP-grasp-type ligase (mdnB and mdnC) and genes,
which encode an ABC transporter (mdnE) and a N-acetyltransferase of the GNAT family (mdnD) (Ziemert et al.,
2008).



The following BioBrick mdnE encodes an ABC transporter (Ziemert et al., 2008).



Because this BioBrick is a RF10 expression part the adenine of mdnE gene start codon is part of the XbaI
recognition site.

Notes

This BioBrick was built by PCR using the following PCR primers

Forward primer: TAAATGAATTCGCGGCCGCTTCTAGATGCCTCAATATACTACTAAAC

Reverse primer: ATTTCTGCAGCGGCCGCTACTAGTACTATATTCTCACCCATTTTAAG

To insert mdnE in the vector pSB1C3, the resulting PCR product and the vector were digested with the restriction enzymes EcoRI and SpeI.



Because this BioBrick is a RF10 expression part the adenine of mdnE gene start codon is part of the XbaI recognition site.

Source

The BioBrick mdnE as a part of the microviridin gene (mdn) cluster was isolated from Microcystis aeruginosa strain NIES-843.

Sequence Annotation Location Component / Role(s)
mdnE
stop
start
1,2062
2060,2062
1,2
CDS feature/cds
stop_codon feature/stop
start_codon feature/start
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K627005/1