Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lauren Cole
Date created: 2011-09-27 11:00:00
Date modified: 2015-09-18 05:19:06
Ho1-PcyA-cph8, regulatory system
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K632003_sequence (Version 1) |
Description
Endogenous heme is converted into biliverdin by Phycocyanobilin:ferredoxin oxidoreductase (pcyA). The biliverdin is then converted by Heme oxigenase (ho1) to phycocyanobilin (PCB), which associates with a chimeric light receptor, cph8 which is the fusion product of Cph1 and Envz-OmpR, obtained from BBa_I15010 (http://partsregistry.org/wiki/index.php?title=Part:BBa_I15010). The PCB and cph8 complex phosphorylates the regulatory protein OmpR, which can then activate the OmpC promoter (pompC) to activate the expression of a desired gene. Stimulation of the cph8 component with red light (~660 nm) inhibits autophosporylation of the receptor which can be used to produced a differential output of a desired gene.Notes
Ho1 and pcyA were put under the same inducible promoter, and cph8 was put under a separate inducible promoter.Source
Heme oxigenase (ho1) was obtained from a pMOD plasmid library vector. Phycocyanobilin:ferredoxin oxidoreductase (pcyA) was obtained from genomic DNA from Synechocystis sp. PCC 6803. Cph8 from BioBrick: BBa_I15010.| Access | Instance | Definition |
| public public public | BBa_I15010 BBa_I15009 BBa_I15008 | BBa_I15010 BBa_I15009 BBa_I15008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I15010 BBa_I15009 BBa_I15008 | 1,2263 2270,3044 3051,3801 | BBa_I15010 BBa_I15009 BBa_I15008 |