Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Harvey
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:57
Arabinose inducible TorA-Reflectin A1-sfGFP generator
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K638403_sequence (Version 1) |
Description
This construct is a reflectin A1 protein with a TorA signal peptide on the N terminus, and superfolder GFP (sfGFP) fused at the C-terminus. The reflectin A1 sequence is codon-optimized for E coli. The TorA signal peptide has been shown to allow export of GFP via the twin arginine pathway. The pBAD promoter is inducible with arabinose.Notes
NASource
This construct was assembled from its parts using Gibson Assembly.The reflectin A1 sequence was kindly provided by Dr Wendy Crookes-Goodson.
The TorA tag was synthesised in two overlapping pieces, which were combined by PCR.
| Sequence Annotation | Location | Component / Role(s) |
| AraC BBa_I0500 PC Pbad BBa_B0034 conserved BBa_K638402 reflectin A1 start BBa_I746916 superfolder GFP coding region stop BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,1079 1,1210 1043,1114 1080,1210 1219,1230 1223,1226 1237,1365 1366,2424 2425,2427 2425,3144 2425,3144 3139,3144 3153,3232 3164,3207 3241,3281 3248,3277 3278,3281 3274,3274 | feature/misc sequence_feature feature/BioBrick engineered_region feature/promoter promoter promoter feature/promoter feature/BioBrick engineered_region conserved_region feature/conserved feature/BioBrick engineered_region CDS feature/cds start_codon feature/start engineered_region feature/BioBrick CDS feature/protein feature/stop stop_codon feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop |