| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K641008_sequence (Version 1)
|
Description
This part is a general reporter for any ToxR system. When ToxR dimerizes it binds to Pctx and activates Transcription of genes downstream of Pctx. By putting ffGFP downstream of Pctx the strength of the ToxR construct dimerization is reported by a corresponding strength of GFP expression.
Notes
The only design considerations were tuning an rbs to the ffGFP so that expression levels could clearly be distinguished.
Source
Pctx is a promoter from a virulence island in Vibrio cholerae. The ffGFP is a widely used purely synthetic protein.
