Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wilson Lam, Matthew Orton
Date created: 2011-09-22 11:00:00
Date modified: 2015-05-08 01:12:58
cI repressor tagged with yBFP and a VP16 activation domain
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K642006_sequence (Version 1) |
Description
CI lambda repressor tagged upstream with yeast codon optimized blue fluorescent protein and an rtTA activation domain.Notes
Both cI lambda and yeast codon optimized BFP were designed without a stop codon to enable the construction of a fusion protein with BFP and the rtTA activation domain.Source
The cI lambda repressor was PCR amplified from a plasmid source of a biobrick () that was submitted last year by the team. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. Gene DNA synthesis. The rtTA activation domain was PCR amplified from a plasmid obtained by our host lab.| Sequence Annotation | Location | Component / Role(s) |
| CI ORF XbaI/SpeI mixed site BFP ORF XbaI/SpeI mixed site VP16 Activation domain | 1,713 714,719 720,1418 1419,1424 1425,1810 | feature/cds CDS feature/misc sequence_feature feature/cds CDS sequence_feature feature/misc feature/cds CDS |