Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alex Bina
Date created: 2011-10-22 11:00:00
Date modified: 2015-05-08 01:12:59
RecA1
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K648104_sequence (Version 1) |
Description
RecA is a protein used by E. coli to repair and maintain DNA. Many normal laboratory strains of E. coli, for example DH10B, are mutants capable of an inactivated form of RecA known as RecA1. RecA1 is deficient in all known function of the RecA gene specifically in ATPase activity, binding with DNA in the presence of ATP, and changing conformation in the presence of ATP and repressor cleavage. In order to restore RecA1 to RecA and to reduce its recombination ability while maintaining its repressor cleavage activity a series of mutations was made.This strain of RecAI was mutated so that the EcoRI and PstI restriction sites were removed so that this part could be placed in BioBrick format.
Notes
Needed to remove the restriction sites from the genomic sequence in order to put RecAI into BioBrick plasmid.Source
The RecAI gene comes from the genome of the DH10B Escherichia coli bacteria.| Sequence Annotation | Location | Component / Role(s) |
| PstI mutation EcoRI mutation | 237,237 783,783 | feature/mutation sequence_alteration sequence_alteration feature/mutation |