BBa_K649302

BBa_K649302 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K649302

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Goshi Sugano

Date created: 2011-09-18 11:00:00

Date modified: 2015-05-08 01:13:00

rocF-Arg box

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K649302_sequence (Version 1)

Description

rocF is cording region of Arginase.
ArgBox is operator sequence of ArgI(E. coli).

These were obtaind by colony-PCR, and ligated into pTrc99A.
rocF had 2 EcoRI sites, so we changed these sites on pTrc99A.

At last, backbone was changed to pSB1C3.

Notes

None.

Source

rocF : Bacillus sublitis 168

ArgBox: Escherichia coli MG1655

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rocF	3,890	CDS, feature/protein
ArgBox	899,978	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K649302/1

Member of these Collections

• iGEM Parts Registry