Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ernesto G??mez
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:13:01
IPTG inducible Expression Platform
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K653003_sequence (Version 1) |
Description
We take advances in genetic engineering as synthetic biology, as viable solution to oil pollution cleanup. But for do this; we need to design and build an expression platform that works into our modified E.coli.For this, We choose four standardized biological parts from the Registry, those parts are: IPTG inducible promoter, Ribosomal Binding Site (RBS), GFP Reporter and Double Terminator; Were selected using rationale from molecular biology and genetic engineering to enable us to build up rationally-engineered, our expression platform.
For the iGEM 2011, we will integrate and assembly those parts (P+RBS+GFP+T) along with our BioBrick part using the 3A Assembly Method, to bring up an expression device (P+RBS+rhlAB_BB+GFP+T) for rhamnolipid production in E. coli.
Notes
Those parts were assembled following the 3A assembly method.Source
IPTG inducible promoter with RBShttp://partsregistry.org/wiki/index.php?title=Part:BBa_J04500
GFP reporter plus Double Terminator
http://partsregistry.org/wiki/index.php/Part:BBa_K653001
| Access | Instance | Definition |
| public public | BBa_J04500 BBa_K653001 | BBa_J04500 Reporter + |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K653001 | 1,220 227,1083 | BBa_J04500 Reporter + |