Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cody Tramp
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:13:02
RFP Cloning Control pSB1A3_IntC6803
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K654095_sequence (Version 1) |
Description
This is the base pSB1A3_IntC6803 Synechocystis sp. PCC 6803 cloning integration plasmid, with an RFP expression device in the BioBrick site. This makes cloning constructs into the plasmid easier, as the RFP is a negative control. Simply digest this plasmid and your desired construct with EcoRI and PstI, and then ligate the construct into the plasmid backbone. Any non-recombinant plasmids will appear visibly red/pink on the plates, allowing for easy selection of successful transformants.Notes
noneSource
existing parts| Sequence Annotation | Location | Component / Role(s) |
| J23100 Controlled RFP Expression Device pSB1A3_IntC6803 (K390300) Integration Plasmid | 1,769 770,6762 | feature/BioBrick engineered_region feature/misc sequence_feature |