BBa_K669005

BBa_K669005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K669005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Silvia J Ca??as

Date created: 2011-09-27 11:00:00

Date modified: 2015-05-08 01:13:03

CBP + promoter

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K669005_sequence (Version 1)

Description

Chitooligosaccharide-Binding Protein (CBP) is a cytoplasmatic protein that regulates the transcriptional activity of the ChiS sensor.

When chitin is present in the external media, CBP binds with GlcNAc oligomers (chitin degradation products) and liberates the sensor for the up-regulation of the chitinolitic degradation cascade in Vibrio fischeri ES114.

Notes

Amplified by PCR from V. fischeri ES114.
Due to the presence an internal of PstI restriction site, this part was first inserted in pGEM T Easy to facilitate its construction.

Source

Vibrio fischeri ES114

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CBP promoter	1,84	feature/promoter, promoter
CBP	85,1758	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K669005/1

Member of these Collections

• iGEM Parts Registry