BBa_K676007

BBa_K676007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K676007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kheng Tee Ng
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:13:04

Gyrase A



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K676007_sequence (Version 1)

Description

This is the wild type Gyr A ORF (2627bp) from E.coli , the GyrA subunit is responsible for catalysing the breakage and resealing of DNA strands . A full functional gyrase consist of two GyrA and two GyrB . Gyr A has important tyr122 residue which is involved in forming the phosphotyrosine linkage with the 5' end of the DNA strand in the active site. The full structure of the gyrase is modelled and can be found in our wiki.

Notes

This is the E.coli GyrA ORF with a Pst 1 restriction site at the 1212th bp position. SIte directed mutagenesis should be done to remove the site.

Source

E.coli genome

Sequence Annotation Location Component / Role(s)
start
1
Gyrase A
BBa_K676007
1,3
2626,2628
1,2628
1,2628
feature/start start_codon
feature/stop stop_codon
feature/cds CDS
engineered_region feature/BioBrick
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K676007/1